Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Aspirin on Fractalkine in Rats with Pulmonary Embolism

Ling-Cong Wang¹ , Jian-Nong Wu¹, Guo-Lian Xia¹, Wei-Mao¹, Rong-Biao Ying², Li-Quan Huang¹, Rong-Lin Jiang¹

¹Department Intensive Care Unit, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006; ²Department of Surgical Oncology, Tumor Hospital of Taizhou, Wenling 317502, China.

For correspondence:- Ling-Cong Wang Email: wlc501@126.com

Received: 6 March 2013 Accepted: 30 March 2014 Published: 23 May 2014

Citation: Wang L, Wu J, Xia G, Wei-Mao , Ying R, Huang L, et al. Effect of Aspirin on Fractalkine in Rats with Pulmonary Embolism. Trop J Pharm Res 2014; 13(5):753-760 doi: 10.4314/tjpr.v13i5.15

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of aspirin on fractalkine (FKN) in rats with pulmonary embolism (PE).

Methods: Sprague Dawley rats were divided into control group, sham operation group, PE model group and PE + aspirin group. PE was established by injecting self-embolus into the right jugular vein of the rats. Aspirin was administered orally 1 day and 40 min before PE surgery, and daily thereafter. At 4 and 72 h following embolism, the rat lung tissues were obtained for hematoxylin-eosin (HE) staining as well as measurement of mRNA expression of FKN, TNF-α and IL-1β. Additionally, serum FKN, IL-8, TNF-α, and IL-1β were measured by enzyme linked immunosorbent assay (ELISA).

Results: The serum levels of FKN, IL-8, TNF-α and IL-1β were significantly decreased by treatment with aspirin compared with the PE group (p < 0.05). Furthermore, mRNA expressions of lung FKN, TNF-α and IL-1β in PE group were markedly decreased by treatment with aspirin compared with that in PE group. PE-induced lung injury was alleviated by treatment with aspirin based on the results of pathological examination.

Conclusion: Aspirin has protective effects against PE-induced lung injuries, which is probably mediated by the suppression of the expression of IL-8, TNF-α, IL-1β, and FKN.

Keywords: Aspirin, Pulmonary embolism, Lung injury, Fractalkine